Single-cell Proteomics
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Droplet proteomic sample preparation (nPOP)

 

bioRxiv Preprint nPOP @ protocols.io Q&A

nano-ProteOmic droplet sample Preparation (nPOP) method for single-cell proteomics by mass-spectrometry

 

Introduction Advantages of nPOP Applications Videos

Introduction

The nano-ProteOmic sample Preparation (nPOP) uses piezo acoustic dispensing to isolate individual cells in 300 picoliter volumes and performs all subsequent preparation steps in small droplets on a fluorocarbon-coated slide. This design enables simultaneous sample preparation of thousands of single cells, including lysing, digesting, and labeling individual cells in volumes below 20 nl.

Advantages of nPOP

  1. nPOP can prepare thousands of single cells in a single batch
    • This minimizes the # of batches and associated technical variation
  2. nPOP uses only mass-spec compatible chemicals and volumes below 20 nl
    • This results in very low contamination background
  3. nPOP droplet layouts are computationally programmable and thus very flexible
    • Thus nPOP can be used for all multiplexed or label-free workflows without specialized consumables
  4. nPOP uses accessible consumables and small amounts of labels
    • This keeps costs down to about 10-20 cents / single cell.

nPOP uses the CellenONE instrument, which is commercially available. Without access to CellenONE, one may use mPOP, which can prepares a few hundred single cells in parallel. Both mPOP and nPOP are fully compatible with all mass-spectrometry methods described in this portal.

 

Applications & data

 

 

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