Analysis of single-cell proteomics data

 

This section organizes computational pipelines for the analysis of single-cell proteomics data. These pipelines can be used with data generated from various methods for single-cell proteomics.


 

SCoPE2 pipeline

The pipeline was developed to process the SCoPE2 data from Spech et al., 2019, but with small modifications it has been used with plexDIA and pSCoPE data as well.

 

Increasing confident peptide identifications

DART-ID implements principled Bayesian frameworks for global retention time (RT) alignment and for incorporating RT estimates towards improved confidence estimates of peptide-spectrum-matches.

 

Visualizing LC-MS/MS data

DO-MS is an an interactive open source platform for their interactive visualization and analysis of LC-MS/MS data: Data-driven Optimization of MS (DO-MS)


Community guidelines and recommendations

Analyzing proteins from single cells by tandem mass spectrometry (MS) has become technically feasible. While such analysis has the potential to accurately quantify thousands of proteins across thousands of single cells, the accuracy and reproducibility of such results may be undermined by numerous factors affecting experimental design, sample preparation, data acquisition, and data analysis. Establishing community guidelines and standardized metrics will enhance rigor, data quality, and alignment between laboratories. The community has proposed best practices, quality controls, and data reporting guidelines to assist in the broad adoption of reliable quantitative workflows for single-cell proteomics.

Best practices »


 

Pipelines by colleagues

  • Chris Vanderaa and Laurent Gatto have developed a Bioconductor package scp for manipulating, processing, and analyzing mass spectrometry-based single-cell proteomics data. The package implemented workflows from the SCoPE2 pipeline using the ‘QFeatures’ package and added new functions.
  • Erwin Schoof and colleagues developed SCeptre, a Python pipeline used for processing single-cell proteomics data generated using an isobaric carrier experimental design.