Methods for single-cell proteomics
- Shotgun single-cell proteomics
- Prioritized single-cell proteomics
- Parallel analysis of both single cells and peptides
This section organizes mass spectrometry methods for single-cell protein analysis developed in the Slavov Laboratory. The methods are organized by the year of first publication. Each method links to datasets generated by the method, which are organized in the data section of this web portal. All methods label single cells with mass tags (barcodes), which allows simultaneous analysis of multiple cells in parallel and thus facilitates scaling up throughput and reducing the cost per single cell.
Shotgun single-cell proteomics
Prioritized single-cell proteomics
Parallel analysis of both single cells and peptides
plexDIA quantifies about a thousand proteins per single cell while parallelizing the analysis of both single cells and peptides. It does not use a carrier sample.