Article: Leduc et al, 2021

Single-cell proteomics method: SCoPE2

Sample preparation method: nPOP

Model systems: Cell lines of monocytes (U937 cells) and Hela cells.

RAW Data Processed Data Videos

Application to the cell division cycle

Single-cell protein analysis using nPOP classified cells by cell type and by cell cycle phase. Furthermore, the data allowed us to quantify the covariation between cell cycle protein markers and thousands of proteins. Based on this covariation, we identify cell cycle associated proteins and functions that are shared across cell types and those that differ between cell types.

Raw Data from experiments benchmarking nPOP

• MassIVE Repository:
  • http: MSV000082841
  • ftp:   MSV000082841
  • http: MSV000088167
  • ftp:   MSV000088167

   

   

Processed Data from experiments benchmarking nPOP

• Peptides-raw.csv
  • Peptides x single cells at 1% FDR. The first columns list the peptide sequences and each subsequent column corresponds to a single cell. Peptide identification is based on spectra analyzed by MaxQuant and is enhanced by using DART-ID to incorporate retention time information. See Specht et al., 2019 for details.

• Proteins-processed.csv
  • Proteins x single cells at 1% FDR, imputed and batch corrected.

• Cells.csv
  • Annotation x single cells. Each column corresponds to a single cell and the rows include relevant metadata, such as, cell type, measurements from the isolation of the cell, and derivative quantities, i.e., rRI, CVs, reliability. This file corresponds to Proteins-processed.csv and Peptides-raw.csv files.

• HeLa-proteins.csv
  • Proteins x single cells for HeLa cells at 1% FDR, non imputed and z-scored.

• U-937-proteins.csv
  • Proteins x single cells for U-937 cells at 1% FDR, non mputed and z-scored.

• CellCycle-Proteins.csv
  • A list of cell cycle dependent proteins used in analysis. Cell cycle protein and phases information was used from Whitfield et al, 2002.

Recorded video presentations