Article: Leduc et al, 2022

Single-cell proteomics method: pSCoPE

Sample preparation method: nPOP

Model systems: Cell lines of monocytes (U937 cells) and melanoma cells.

Supplementary tables RAW Data Code Protocol Processed Data Videos

Supplementary tables

• Supp Table 1: Data Organization
  • A table describing all data from nPOP paper including the MassIVE repository, proccessed data, and supplmental data
• Supp Software 2: CellenONE instrument files
  • All CellenONE software files needed for performing nPOP
• Supp Video 3: Video of Pickup
  • A video of the cellenONE gathering samples off slides and dispensing into a 384 well plate for subsequent analysis
• Supp Data 4: Meta data
  • Meta data for pSCoPE and plexDIA runs
• Supp Data 5: pSCoPE DO-MS report
  • Protein set enrichment analysis between melanoma sub-populations
• Supp Table 6: CDC analysis
  • Cell cycle analysis containing differential proteins from bulk, proteins used to create CDC markers, proteins that correlate significantly to CDC phases, and protein set enrichment computed from CDC correlations
• Supp Table 7: Melanoma subopulation analysis
  • Differentially abundant proteins between melanoma sub-populations and protein set enrichment analysis
• Supp Figures 8: Supplemental figures
  • The three supplemental figures from the paper
• Supp Data 9: Proccessed data
  • Normalized and imputed protein x single cell matricies for pSCoPE and plexDIA data

   

   

Raw Data from experiments benchmarking nPOP

• MassIVE Repository:
  • Description of Repository
  • http: MSV000089159
  • ftp:   MSV000089159

   

   

Processed Data from experiments benchmarking nPOP

• Processed Data
  • This folder contains all processed data necessary for performing the analysis, as well as outputs from analysis

   

   

Recorded video presentations