Single Cell Proteomics

Methods & Data

Quantifying proteins in single cells with mass spectrometry

Perspectives Reviews Highlights Resources

Each cell performs its biological functions by changing the abundance, localization, modifications and interactions of its proteins. Analyzing these processes at single-cell resolution requires quantifying proteins in single cells, which is the focus of this web portal. Its pages include single-cell proteomics methods, computational analysis tools, and data and metadata from single mammalian cells, both primary cells and cell lines.

Methods   Data   Sample prep   Computation   Biology

Perspectives

• Extending the sensitivity, consistency and depth of single-cell proteomics, Nature Methods
• Sampling the proteome by emerging single-molecule and mass spectrometry methods, Nature Methods
• Strategies for increasing the depth and throughput of protein analysis by plexDIA, Journal of Proteome Research
• Framework for multiplicative scaling of single-cell proteomics, Nature Biotechnology
• Learning from natural variation across the proteomes of single cells, PLOS Biology
• Driving Single Cell Proteomics Forward with Innovation, Journal of Proteome Research
• Unpicking the proteome in single cells, Science
• Transformative Opportunities for Single-Cell Proteomics, Journal of Proteome Research

Reviews

• Scaling up single-cell proteomics, Molecular and Cellular Proteomics
• Single-cell protein analysis by mass spectrometry, Current Opinion in Chemical Biology
• Single cell protein analysis for systems biology, Essays in biochemistry

Highlights

• Single-Cell Proteomics Bypasses Bottlenecks, Sways Skeptics, GEN
• Untangling the Complexities of Single Cell Protein Analysis, Precision Medicine
• Uncovering Proteomic Patterns One Cell at a Time, GEN
• Highlight of plexDIA, Nature Methods
• Highlight of SCoPE-MS and mPOP, C&EN
• Highlight of SCoPE-MS and nPOP, Nature
• Highlight of SCoPE2, Nature Methods
• Technology feature, Nature Methods
• Single-cell proteomics for the Human Cell Atlas, NEU News

Community Resources

• White Paper: Performing, benchmarking, and reporting single-cell proteomics experiments, Nat Methods 20, 375–386 (2023) PDF, preprint, Code, Guidelines website
• Protocols & Tutorials: Single-cell proteomics protocols
• Dissemination: Disseminating Portal
• Scientific Meeting: Single-cell proteomics conference
  • Recorded research presentations

About

This site provides resources and data for single-cell proteomics from the Slavov Laboratory

The research reported here has been supported by funding from the NIH Director’s Award by an Allen Distinguished Investigator Award from the Paul G. Allen Frontiers Group and by CZI.