Single Cell Proteomics
Methods & Data
Quantifying proteins in single cells with mass spectrometry
Perspectives Reviews Highlights Resources
Each cell performs its biological functions by changing the abundance, localization, modifications and interactions of its proteins. Analyzing these processes requires quantifying proteins in single cells, which is the focus of this web portal. Its pages include both single-cell proteomics methods and data from single mammalian cells, both primary cells and cell lines.
Methods Data Sample prep Computation Biology
Perspectives
- Strategies for increasing the depth and throughput of protein analysis by plexDIA, Journal of Proteome Research
- Framework for multiplicative scaling of single-cell proteomics, Nature Biotechnology
- Learning from natural variation across the proteomes of single cells, PLOS Biology
- Driving Single Cell Proteomics Forward with Innovation, Journal of Proteome Research
- Unpicking the proteome in single cells, Science
- Transformative Opportunities for Single-Cell Proteomics, Journal of Proteome Research
Reviews
- Single-cell protein analysis by mass spectrometry, Current Opinion in Chemical Biology
- Single cell protein analysis for systems biology, Essays in biochemistry
- Scaling up single-cell proteomics, Molecular and Cellular Proteomics
Highlights
- Untangling the Complexities of Single Cell Protein Analysis, Precision Medicine
- Uncovering Proteomic Patterns One Cell at a Time, GEN
- Highlight of plexDIA, Nature Methods
- Highlight of SCoPE-MS and mPOP, C&EN
- Highlight of SCoPE-MS and nPOP, Nature
- Highlight of SCoPE2, Nature Methods
- Technology feature, Nature Methods
- Single-cell proteomics for the Human Cell Atlas, NEU News
Community Resources
- White Paper: Performing, benchmarking, and reporting single-cell proteomics experiments, Nat Methods 20, 375–386 (2023) PDF, preprint, Code, Guidelines website
- Protocols & Tutorials: Disseminating Portal
About
This site provides resources and data for single-cell proteomics from the Slavov Laboratory
The research reported here has been supported by funding from the NIH Director’s Award by an Allen Distinguished Investigator Award from the Paul G. Allen Frontiers Group and by CZI.