Single Cell Proteomics

Methods & Data

Quantifying proteins in single cells with mass spectrometry

Perspectives Reviews Highlights

Each cell performs its biological functions by changing the abundance, localization, modifications and interactions of its proteins. Analyzing these processes requires quantifying proteins in single cells, which is the focus of this web portal. Its pages include both single-cell proteomics methods and data from single mammalian cells, both primary cells and cell lines.

Methods   Data   Sample prep   Computation   Biology

Perspectives

• Framework for multiplicative scaling of single-cell proteomics, Nature Biotechnology
• Learning from natural variation across the proteomes of single cells, PLOS Biology
• Driving Single Cell Proteomics Forward with Innovation, Journal of Proteome Research
• Unpicking the proteome in single cells, Science
• Transformative Opportunities for Single-Cell Proteomics, Journal of Proteome Research

Reviews

• Single-cell protein analysis by mass spectrometry, Current Opinion in Chemical Biology
• Single cell protein analysis for systems biology, Essays in biochemistry
• Scaling up single-cell proteomics, Molecular and Cellular Proteomics

Highlights

• Highlight by C&EN
• Highlight by Nature
• Highlight by Nature Methods
• Technology feature at Nature Methods
• Single-cell proteomics for the Human Cell Atlas

About

This site provides resources and data for single-cell proteomics from the Slavov Laboratory

The research reported here has been supported by funding from the NIH Director’s Award by an Allen Distinguished Investigator Award from the Paul G. Allen Frontiers Group and by CZI.