Article: Leduc et al, 2022
- Code available at: github.com/SlavovLab/nPOP
- Peer reviewed article: Leduc A, Huffman RG, Cantlon J, Khan S, Slavov N, Exploring functional protein covariation across single cells using nPOP Genome Biol 23, 261 10.1186/s13059-022-02817-5
- Perspective: Slavov N., Learning from natural variation across the proteomes of single cells, PLoS Biology 20(1): e3001512
Single-cell proteomics methods: pSCoPE and plexDIA
Sample preparation method: nPOP
Model systems: Cell lines of monocytes (U937 cells) and melanoma cells (WM989-A6-G3).
Supplementary tables RAW Data Code Protocol Processed Data Videos
Supplementary tables
- Supp Table 1: Data Organization
- A table describing all data from nPOP paper including the MassIVE repository, processed data, and supplemental data
- Supp Software 2: CellenONE instrument files
- All CellenONE software files needed for performing nPOP
- Supp Video 3: Video of Pickup
- A video of the cellenONE gathering samples off slides and dispensing into a 384 well plate for subsequent analysis
- Supp Data 4: Meta data
- Meta data for pSCoPE and plexDIA runs
- Supp Data 5: pSCoPE DO-MS report
- Protein set enrichment analysis between melanoma sub-populations
- Supp Table 6: CDC analysis
- Cell cycle analysis containing differential proteins from bulk, proteins used to create CDC markers, proteins that correlate significantly to CDC phases, and protein set enrichment computed from CDC correlations
- Supp Table 7: Melanoma subpopulation analysis
- Differentially abundant proteins between melanoma sub-populations and protein set enrichment analysis
- Supp Figures 8: Supplemental figures
- Supplemental figures from the paper
- Supp Data 9: Processed data
- Searched data, processed data, and additional data files for completing analysis performed Here
Raw Data from experiments benchmarking nPOP
- MassIVE Repository:
Processed Data from experiments benchmarking nPOP
- Processed Data
- This folder contains all processed data necessary for performing the analysis, as well as outputs from analysis
Recorded video presentations